Molecular characterization of commonly used cell lines for bone tumor research: A trans‐European EuroBoNet effort
Identifieur interne : 000C21 ( Main/Exploration ); précédent : 000C20; suivant : 000C22Molecular characterization of commonly used cell lines for bone tumor research: A trans‐European EuroBoNet effort
Auteurs : Laura Ottaviano [Allemagne] ; Karl-Ludwig Schaefer [Allemagne] ; Melanie Gajewski [Allemagne] ; Wolfgang Huckenbeck [Allemagne] ; Stefan Baldus [Allemagne] ; Uwe Rogel [Allemagne] ; Carlos Mackintosh [Espagne] ; Enrique De Alava [Espagne] ; Ola Myklebost [Norvège] ; Stine H. Kresse [Norvège] ; Leonardo A. Meza-Zepeda [Norvège] ; Massimo Serra [Italie] ; Anne-Marie Cleton-Jansen [Pays-Bas] ; Pancras C. W. Hogendoorn [Pays-Bas] ; Horst Buerger [Allemagne] ; Thomas Aigner [Allemagne] ; Helmut E. Gabbert [Allemagne] ; Christopher Poremba [Allemagne]Source :
- Genes, Chromosomes and Cancer [ 1045-2257 ] ; 2010-01.
Abstract
Usage of cancer cell lines has repeatedly generated conflicting results provoked by differences among subclones or contamination with mycoplasm or other immortal mammalian cells. To overcome these limitations, we decided within the EuroBoNeT consortium to characterize a common set of cell lines including osteosarcomas (OS), Ewing sarcomas (ES), and chondrosarcomas (CS). DNA fingerprinting was used to guarantee the identity of all of the cell lines and to distinguish subclones of osteosarcoma cell line HOS. Screening for homozygous loss of 38 tumor suppressor genes by MLPA revealed deletion of CDKN2A as the most common event (15/36), strictly associated with absence of the CDKN2A (p16) protein. Ten cell lines showed missense mutations of the TP53 gene while another set of nine cell lines showed mutations resulting in truncation of the TP53 protein. Cells harboring missense mutations expressed high levels of nuclear TP53, while cell lines with nonsense mutations showed weak/absent staining for TP53. TP53wt cell lines usually expressed the protein in 2–10% of the cells. However, seven TP53wt osteosarcomas were negative for both mRNA and protein expression. Our analyses shed light on the correlation between immunohistochemical and genetic data for CDKN2A and TP53, and confirm the importance of these signaling pathways. The characterization of a substantial number of cell lines represents an important step to supply research groups with proven models for further advanced studies on tumor biology and may help to make results from different laboratories more comparable. © 2009 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/gcc.20717
Affiliations:
- Allemagne, Espagne, Italie, Norvège, Pays-Bas
- District de Düsseldorf, District de Leipzig, Hollande-Méridionale, Rhénanie-du-Nord-Westphalie, Saxe (Land), Østlandet
- Düsseldorf, Leipzig, Leyde, Oslo
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Meza-Zepeda</name><affiliation wicri:level="3"><country xml:lang="fr">Norvège</country><wicri:regionArea>Department of Tumor Biology, The Norwegian Radium Hospital, Rikshospitalet University Hospital, Oslo</wicri:regionArea><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Serra, Massimo" sort="Serra, Massimo" uniqKey="Serra M" first="Massimo" last="Serra">Massimo Serra</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Laboratorio di Ricerca Oncologica, Istituti Ortopedici Rizzoli, Bologna</wicri:regionArea><wicri:noRegion>Bologna</wicri:noRegion></affiliation></author><author><name sortKey="Cleton Ansen, Anne Arie" sort="Cleton Ansen, Anne Arie" uniqKey="Cleton Ansen A" first="Anne-Marie" last="Cleton-Jansen">Anne-Marie Cleton-Jansen</name><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Pathology, Leiden University Medical Center, Leiden</wicri:regionArea><placeName><settlement type="city">Leyde</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Hogendoorn, Pancras C W" sort="Hogendoorn, Pancras C W" uniqKey="Hogendoorn P" first="Pancras C. W." last="Hogendoorn">Pancras C. W. Hogendoorn</name><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Pathology, Leiden University Medical Center, Leiden</wicri:regionArea><placeName><settlement type="city">Leyde</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Buerger, Horst" sort="Buerger, Horst" uniqKey="Buerger H" first="Horst" last="Buerger">Horst Buerger</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Pathology, University Medical Center Muenster, Muenster</wicri:regionArea><wicri:noRegion>Muenster</wicri:noRegion><wicri:noRegion>Muenster</wicri:noRegion></affiliation></author><author><name sortKey="Aigner, Thomas" sort="Aigner, Thomas" uniqKey="Aigner T" first="Thomas" last="Aigner">Thomas Aigner</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Pathology, University of Leipzig, Leipzig</wicri:regionArea><placeName><region type="land" nuts="1">Saxe (Land)</region><region type="district" nuts="2">District de Leipzig</region><settlement type="city">Leipzig</settlement></placeName></affiliation></author><author><name sortKey="Gabbert, Helmut E" sort="Gabbert, Helmut E" uniqKey="Gabbert H" first="Helmut E." last="Gabbert">Helmut E. 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Cancer</title><idno type="ISSN">1045-2257</idno><idno type="eISSN">1098-2264</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2010-01">2010-01</date><biblScope unit="volume">49</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="40">40</biblScope><biblScope unit="page" to="51">51</biblScope></imprint><idno type="ISSN">1045-2257</idno></series><idno type="istex">E8374F395D7E218EE22C21CB4F7C9DACF7D1C4D9</idno><idno type="DOI">10.1002/gcc.20717</idno><idno type="ArticleID">GCC20717</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1045-2257</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Usage of cancer cell lines has repeatedly generated conflicting results provoked by differences among subclones or contamination with mycoplasm or other immortal mammalian cells. To overcome these limitations, we decided within the EuroBoNeT consortium to characterize a common set of cell lines including osteosarcomas (OS), Ewing sarcomas (ES), and chondrosarcomas (CS). DNA fingerprinting was used to guarantee the identity of all of the cell lines and to distinguish subclones of osteosarcoma cell line HOS. Screening for homozygous loss of 38 tumor suppressor genes by MLPA revealed deletion of CDKN2A as the most common event (15/36), strictly associated with absence of the CDKN2A (p16) protein. Ten cell lines showed missense mutations of the TP53 gene while another set of nine cell lines showed mutations resulting in truncation of the TP53 protein. Cells harboring missense mutations expressed high levels of nuclear TP53, while cell lines with nonsense mutations showed weak/absent staining for TP53. TP53wt cell lines usually expressed the protein in 2–10% of the cells. However, seven TP53wt osteosarcomas were negative for both mRNA and protein expression. Our analyses shed light on the correlation between immunohistochemical and genetic data for CDKN2A and TP53, and confirm the importance of these signaling pathways. The characterization of a substantial number of cell lines represents an important step to supply research groups with proven models for further advanced studies on tumor biology and may help to make results from different laboratories more comparable. © 2009 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Espagne</li><li>Italie</li><li>Norvège</li><li>Pays-Bas</li></country><region><li>District de Düsseldorf</li><li>District de Leipzig</li><li>Hollande-Méridionale</li><li>Rhénanie-du-Nord-Westphalie</li><li>Saxe (Land)</li><li>Østlandet</li></region><settlement><li>Düsseldorf</li><li>Leipzig</li><li>Leyde</li><li>Oslo</li></settlement></list><tree><country name="Allemagne"><noRegion><name sortKey="Ottaviano, Laura" sort="Ottaviano, Laura" uniqKey="Ottaviano L" first="Laura" last="Ottaviano">Laura Ottaviano</name></noRegion><name sortKey="Aigner, Thomas" sort="Aigner, Thomas" uniqKey="Aigner T" first="Thomas" last="Aigner">Thomas Aigner</name><name sortKey="Baldus, Stefan" sort="Baldus, Stefan" uniqKey="Baldus S" first="Stefan" last="Baldus">Stefan Baldus</name><name sortKey="Buerger, Horst" sort="Buerger, Horst" uniqKey="Buerger H" first="Horst" last="Buerger">Horst Buerger</name><name sortKey="Gabbert, Helmut E" sort="Gabbert, Helmut E" uniqKey="Gabbert H" first="Helmut E." last="Gabbert">Helmut E. Gabbert</name><name sortKey="Gajewski, Melanie" sort="Gajewski, Melanie" uniqKey="Gajewski M" first="Melanie" last="Gajewski">Melanie Gajewski</name><name sortKey="Huckenbeck, Wolfgang" sort="Huckenbeck, Wolfgang" uniqKey="Huckenbeck W" first="Wolfgang" last="Huckenbeck">Wolfgang Huckenbeck</name><name sortKey="Poremba, Christopher" sort="Poremba, Christopher" uniqKey="Poremba C" first="Christopher" last="Poremba">Christopher Poremba</name><name sortKey="Poremba, Christopher" sort="Poremba, Christopher" uniqKey="Poremba C" first="Christopher" last="Poremba">Christopher Poremba</name><name sortKey="Rogel, Uwe" sort="Rogel, Uwe" uniqKey="Rogel U" first="Uwe" last="Rogel">Uwe Rogel</name><name sortKey="Schaefer, Karl Udwig" sort="Schaefer, Karl Udwig" uniqKey="Schaefer K" first="Karl-Ludwig" last="Schaefer">Karl-Ludwig Schaefer</name><name sortKey="Schaefer, Karl Udwig" sort="Schaefer, Karl Udwig" uniqKey="Schaefer K" first="Karl-Ludwig" last="Schaefer">Karl-Ludwig Schaefer</name></country><country name="Espagne"><noRegion><name sortKey="Mackintosh, Carlos" sort="Mackintosh, Carlos" uniqKey="Mackintosh C" first="Carlos" last="Mackintosh">Carlos Mackintosh</name></noRegion><name sortKey="De Alava, Enrique" sort="De Alava, Enrique" uniqKey="De Alava E" first="Enrique" last="De Alava">Enrique De Alava</name></country><country name="Norvège"><region name="Østlandet"><name sortKey="Myklebost, Ola" sort="Myklebost, Ola" uniqKey="Myklebost O" first="Ola" last="Myklebost">Ola Myklebost</name></region><name sortKey="Kresse, Stine H" sort="Kresse, Stine H" uniqKey="Kresse S" first="Stine H." last="Kresse">Stine H. Kresse</name><name sortKey="Meza Epeda, Leonardo A" sort="Meza Epeda, Leonardo A" uniqKey="Meza Epeda L" first="Leonardo A." last="Meza-Zepeda">Leonardo A. Meza-Zepeda</name></country><country name="Italie"><noRegion><name sortKey="Serra, Massimo" sort="Serra, Massimo" uniqKey="Serra M" first="Massimo" last="Serra">Massimo Serra</name></noRegion></country><country name="Pays-Bas"><region name="Hollande-Méridionale"><name sortKey="Cleton Ansen, Anne Arie" sort="Cleton Ansen, Anne Arie" uniqKey="Cleton Ansen A" first="Anne-Marie" last="Cleton-Jansen">Anne-Marie Cleton-Jansen</name></region><name sortKey="Hogendoorn, Pancras C W" sort="Hogendoorn, Pancras C W" uniqKey="Hogendoorn P" first="Pancras C. W." last="Hogendoorn">Pancras C. W. Hogendoorn</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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